|
KMID : 0614619940260030529
|
|
Korean Journal of Gastroenterology
1994 Volume.26 No. 3 p.529 ~ p.540
|
|
|
Detection of Hepatitis B Virus Integrated into Huma Hepatocellular Carcinoma
|
|
|
|
|
Abstract
|
|
|
The insertion of hepatitis B virus(HBV) DNA into the chromosomal DNA of hepatocellular carcinoma(HCC)tissue was identified in most patients tested. X gene locus of HBV in the chromosomal DNA of HCC tissue was detected in 9 out of 11 patients, and
pre S
and S gene loci were detected in 1 and 8 out of 11 patients, respectively. The insertion of P gene locus of HBV into the chromosomal DNA was detected in 6 out of 11 patients, but the insertion of C gene was not detected in any of them. About
400bp
amplified by polymerase chain reaction using an oligonucleotide complementary to pre S gene locus and nonspecific oligo dG as primers revealed that HBV DNA(No. 42 base) was linked to chromosomal DNA. From these results it is concluded that HBV
DNA
was
inserted into the chromosomal DNA of most HCC tissues from the HBV infected patients and that the insertion of X and S gene loci were predominant. The present results also implicate that the single stranded region of HBV genome is frequent
insertion
site and that direct repeat sequences(DR1, DR2) of X gene might involve in the HBV DNA insertion into the chromosomal DNA. (Korean J Gastroenterol 1994 ; 26 : 529-540)
|
|
|
KEYWORD
|
|
|
|
|
|
FullTexts / Linksout information
|
|
|
|
|
Listed journal information
|
|
   
|